Fig. 8.

circAGFG1 promotes cell proliferation and invasion through circAGFG1/miR-195-5p/CCNE1 axis. a and b The cell proliferation and invasion were determined after transfection with indicated vectors, miRNAs or inhibitors by EdU and transwell assays, respectively (magnification, × 100, scale bar, 100 μm). c Relative protein levels of CCNE1 in cells were assessed by IF after transfection (magnification, × 100, scale bar, 100 μm). d and e Relative expression of CCNE1 and downstream cell cycle-related molecules at protein level in cells transfected with indicated vectors, miRNAs or inhibitors was determined by western blot. f Schematic diagram of how circAGFG1 promotes TNBC tumorigenesis and progression. Data were indicated as mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001