Table 1.

Primers used for amplification and sequence analysis of human PAX6

Exon	Forward Sequence 5′-3′	Reverse Sequence 5′-3′
EXON04	AGATCGCCCCAAGAGGTTG	ATCGAGAAGAGCCAAGCAAAC
EXON05	GGTGGTCCTGTTGTCCTTTAT	GGGGTCCATAATTAGCATCGT
EXON06–07	AGCTCTCTACAGTAAGTTCTCA	CCCAGGTACAAAGGAGACAAA
EXON08	TCCGCCCAATTCTCTATCCAA	TACACAACCCTCACATTCCCA
EXON09	GGTGAGGCTGTCGGGATATAA	TCTTTGTACTGAAGATGTGGCA
EXON10–11	TAACTTGGTTCTGGTGGGAAA	CGGAGCAAACAGGTTTAAAGA
EXON11–12	TGCTAACCTGTCCCACCTG	GAAAAGCTCTCAAGGGTGCAG
EXON12–13	AGGCTTGATACATAGGCAGCT	GGACAAGGAAAGCAAGGAGTT
EXON14	TGTATTCCATGTCTGTTTCTCA	GGTACAATACAGGACACAATTG

Note: All primers were amplified using a touchdown protocol beginning at 64 °C, decreasing

by 0.5 °C each cycle, until finishing at a final annealing temperature of 57°°C