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. 2019 Jan 8;19:29. doi: 10.1186/s12885-018-5246-0

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Acquirement of Ago2 complexes from cytoplasm and nucleus of gastric cancer cells. a Isolation of nuclei and cytoplasm. The nuclei and cytoplasm of gastric cancer cells (MGC-803) were isolated and then examined by Western blot analysis. Antibody against β-tubulin, a marker of cytoplasm, or Lamin A/C, a marker of nucleus was indicated on the right. b Co-immunoprecipitation of Ago2 complex of isolated nucleus and cytoplasm. Co-IP was conducted using isolated nucleus or cytoplasm with Ago2-specific antibody. The Co-IP products were detected by Western blot to detect the Ago2 protein