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. 2019 Jan 9;12:11. doi: 10.1186/s13068-018-1351-1

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Transformation of OsGH9B1 and OsGH9B3 produced overexpressed proteins in the transgenic lines. a Constructs used for overexpression of the OsGH9B1 and OsGH9B3 genes. b Gene expression in WT (Nipponbare) plants and four transgenic lines. c Detection of OsGH9B1 and OsGH9B3 proteins in total proteins samples extracted from stem tissues. d The distribution of proteins in soluble protein samples (Sup), plasma membrane protein (PMy, and total protein samples (Res). e Observation of OsGH9B1/B3-eGFP in protoplast, scale bar as 10 μm. f Cellulase activity assay in vitro using total proteins extracted from stem tissues; Student’s t test performed for WT plants and transgenic lines as **p < 0.01 and *p < 0.05