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. 2019 Jan 8;12:2. doi: 10.1186/s13045-018-0689-y

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — a Evaluation by quantitative real-time PCR of mRNA expression of ALP, OCN, and COL1A1 in hTERT-MSC treated for 10 and 14 days with MM1.S exosomes under undifferentiating medium (ctrl) or in osteogenic differentiation medium (diff). b ALP protein release was evaluated by ELISA assay in the conditioned medium of hTERT-MSC treated for 14 days with MM1.S exosomes under undifferentiating medium (ctrl) or in osteogenic differentiation medium (diff). c Quantification of in vitro osteoblast mineralization in the hTERT-MSC treated for 10 and 14 days with MM1.S exosomes under undifferentiating medium (ctrl) or in osteogenic differentiation medium (diff) was evaluated using OsteoImage Mineralization Assay Kit. Values are expressed as fluorescence units (RFU; 492 nm excitation/520 nm emission wavelengths). *Diff vs ctrl (*p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001); ^#Diff+ Exo vs diff (^#p ≤ 0.05; ^##p ≤ 0.01). d Evaluation by quantitative real-time PCR of mRNA expression of ALP, OCN, and COL1A1 in hTERT-MSC treated for 14 days with exosomes isolated from BM plasma of three MM patients in osteogenic differentiation medium (diff). e ALP protein release was evaluated by ELISA assay in the conditioned medium of hTERT-MSC treated for 14 days with exosomes isolated from BM plasma of three MM patients in osteogenic differentiation medium (diff). ^#Diff+ Exo vs diff (^#p ≤ 0.05; ^##p ≤ 0.01; ^###p ≤ 0.001)