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. 2019 Jan 8;20:20. doi: 10.1186/s12864-018-5319-0

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© The Author(s). 2019

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — In vivo validation of the phenotype of a S. Typhimurium ptsN mutant. (a) The TraDIS fitness scores of transposon-insertion mutants of ptsN indicate attenuation in MLNs but not in the ileal wall. (b) Competitive index scores of ST4/74 nal^R ∆ptsN::cat compared to the wild-type ST4/74 nal^R confirmed the phenotype predicted by TraDIS in the ileal wall and MLNs. CIs in the caecal lymph node, liver and spleen show attenuation of ST4/74 nal^R ∆ptsN::cat in these tissues. CIs in faecal samples decreased over time. (c) Bacterial counts confirmed the total challenge dose of 3.07 × 10⁸ CFU of mutant and wild-type bacteria and recovery of viable bacteria per tissue per calf is shown