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. 2019 Jan 8;20:20. doi: 10.1186/s12864-018-5319-0

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© The Author(s). 2019

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — In vivo validation of the phenotype of a S. Typhimurium spvC mutant. (a) The TraDIS fitness scores of transposon-insertion mutatns of spvC indicate attenuation in the ileal wall but not in MLNs. (b) Competitive index scores of ST4/74 nal^R ∆spvC::cat compared to the wild-type ST4/74 nal^R confirmed the phenotype predicted by TraDIS in the ileal wall and MLNs. CIs in the caecal lymph node were similar to those in the ileal wall but CIs in the liver and spleen show no attenuation of ST4/74 nal^R ∆spvC::cat in these tissues. CIs in faecal samples decreased over time. (c) Bacterial counts confirmed the total challenge dose of 7.2 × 10⁸ CFU of mutant and wild-type bacteria and recovery of viable bacteria per tissue per calf is shown