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. 2018 Nov 5;9(1):81–94. doi: 10.1534/g3.118.200726

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Copyright © 2019 Shih et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Characterizing mushroom body subtype drivers. (A-G) Expression pattern of the split-GAL4 driver lines used in this study. Green, the GFP plus anti-GFP immunoreactive signal; magenta, anti-dlg1 immunoreactive signal as a counterstain. The scale bar represents 50μm. (A′-G′) 3D model of the split-GAL4 expression pattern in MB. (A″-G″) Example of the high-resolution membrane-GFP-P2A-nuclear-mCherry dual label reporter (WM for short; see Methods) images used for cell counting. The scale bar represents 20μm. Genotype: MB607B > WM, R19B03-p65.AD/UAS-WM-2; R39A11-GAL4.DBD/+, MB131B > WM, R13F02-p65.AD/UAS-WM-2; R89B01-GAL4.DBD/+, MB370B > WM, R13F02-p65.AD/UAS-WM-2; R41C07-GAL4.DBD/+, MB418B > WM, R26E07-p65.AD/UAS-WM-2; R30F02-GAL4.DBD/+, MB371B > WM, R13F02-p65.AD/UAS-WM-2; R85D07-GAL4.DBD/+, MB185B > WM, R52H09-p65.AD/UAS-WM-2; R18F09-GAL4.DBD/+, and MB594B > WM, R13F02-p65.AD/UAS-WM-2; R58F02-GAL4.DBD/+.