Table 2. Quality Control PCR Interpretation.

Mutant Class	Left Insertion PCR	Right Insertion PCR	WT PCR on Mutant	WT PCR on N2	Conclusion
Precise Edit	Correct	Correct	No product	Correct	Mutant, probably as expected
Imprecise Edit	Correct	Incorrect	No product	Correct	Mutant, probably with rearrangement
Imprecise Edit	Incorrect	Correct	No product	Correct	Mutant, probably with rearrangement
Imprecise Edit	Incorrect	Incorrect	No product	Correct	Mutant, probably with rearrangement
Gene Partially Intact	Any result	Any result	WT product	Correct	Not a null mutant