Figure 5.

The DNA fragment deletion was enhanced using Cas9-vasa 3′-UTR in gonads. (A) the vasa 3′-UTR sequence of tilapia used in this study was listed. (B) vasa 3′-UTR direct GFP mRNA expression in the germ cells. GFP expressing cells indicated the germ cells (indicated by arrowhead). (C) vasa 3′-UTR was inserted into downstream of Cas9 open reading frame. (D) PCR amplification showed successful deletion of the target DNA in the gonad tissue (indicated by arrow). (E) Sequencing of the PCR products further confirmed the deletions. Deletions are marked by dashes. WT, wild type.