Table 1. Fragment deletion of miRNA200a/200b/429a in each mutant induced by CRISPR/Cas9.
| Target | Number of F0 tested | Mutant | Frequency (%) | Fragment deletion frequency (%) | Average deletion frequency (%) | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| #1 | #2 | #3 | #4 | #5 | #6 | #7 | #8 | |||||
| miRNA200a/200b | 20 | 12 | 60 | 5 | 10 | 9 | 11 | 8 | 11 | 6 | 7 | 8.3 |
| miRNA429a | 40 | 10 | 25 | 8 | 7 | 9 | 10 | 12 | 9 | 6 | 7 | 8.4 |
| miRNA200a/200b/429a | 100 | 12 | 12 | 9 | 6 | 8 | 7 | 9 | 9 | 4 | 6 | 7.2 |
| miRNA200a/200b/429a+80 bp ssDNA | 50 | 11 | 22 | 19 | 11 | 8 | 9 | 12 | 16 | 5 | 11 | 11.4* |
Asterisk indicates significant difference between miRNA200a/200b/429a mutants and miRNA200a/200b/429a+80 bp ssDNA mutants (P < 0.05).