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| Group | Founder fish | F1 individuals evaluated | Positive individuals | % of germline transmission |
|---|---|---|---|---|
| 1 (Cas9) | #1 | 52 | 5 | 9.6 |
| #2 | 38 | 3 | 7.9 | |
| #3 | 42 | 5 | 11.9 | |
| #4 | 36 | 2 | 5.5 | |
| Average efficiency | 8.7 | |||
| 2 (Cas9-vasa 3′-UTR) | #5 | 42 | 5 | 11.9 |
| #6 | 33 | 6 | 18.1 | |
| #7 | 38 | 5 | 13.1 | |
| #8 | 24 | 4 | 16.6 | |
| Average efficiency | 14.9* | |||
Asterisk indicates significant difference between group 1 and 2 mutants (P < 0.05).
