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. Author manuscript; available in PMC: 2019 Jan 9.
Published in final edited form as: Cell Rep. 2018 Sep 4;24(10):2784–2794. doi: 10.1016/j.celrep.2018.08.018

Figure 5. Cell Cycle and NADPH Oxidase Regulation in Neutrophil Differentiation.

Figure 5

(A) Flow cytometry analysis of Ki67 expression in bone marrow neutrophil progenitor fractions normalized to isotope control in gray for the three biological replicates.

(B) Schematic of the assembly of the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase enzyme complex on the phagosomal or plasma membrane upon neutrophil activation.

(C) Heatmap representing the expression in log2(FPKM+1) of NADPH complex subunits during neutrophil differentiation.

(D) Respiratory burst in sorted neutrophil progenitors from bone marrow and mature PMN upon stimulation with PMA and PAF/fMLP. Results are expressed as maximal rates in nmol H2O2/min · 106 PMNs and as mean ± SEM from the three biological replicates. t test corrected p values of the consecutive stages are indicated in the figure: *p < 0.1 and **p < 0.01. ns, nonsignificant. The t test corrected p values of all comparisons are reported in Table S5.