Table 1.

Accuracy and precision of DHT, α-diol, and β-diol at LLOQ in six individual lots of mouse sera (n = 5).

Sera	[XN]a (ng/mL)	DHT			α-diol			β-diol
		$\left[\overline{X}\right]$b ± SDc(ng/mL)	CVd (%)	REe (%)	$\left[\overline{X}\right]$ ± SD (ng/mL)	CV(%)	RE (%)	$\left[\overline{X}\right]$ ± SD (ng/mL)	CV(%)	RE (%)
Lot 1	0.0500	0.051 ± 0.002	4	2	0.051 ± 0.001	2	2	0.051 ± 0.002	4	2
Lot 2	0.0500	0.051 ± 0.001	2	2	0.050 ± 0.002	4	0	0.051 ± 0.002	4	2
Lot 3	0.0500	0.049 ± 0.002	4	−2	0.050 ± 0.002	4	0	0.051 ± 0.002	4	2
Lot 4	0.0500	0.051 ± 0.002	4	2	0.052 ± 0.002	4	4	0.049 ± 0.001	2	−2
Lot 5	0.0500	0.052 ± 0.002	4	4	0.051 ± 0.002	4	2	0.049 ± 0.002	4	−2
Lot 6	0.0500	0.052 ± 0.002	4	4	0.049 ± 0.002	4	−2	0.053 ± 0.003	6	6
Mean of 6 lots	0.0500	0.051 ± 0.001	1	2	0.051 ± 0.001	2	1	0.051 ± 0.001	2	1

^a[X_N] = nominal concentration of analyte sera calibrator.

^b$\left[\overline{X}\right]$ = mean value of measured analyte concentration.

^cSD = standard deviation of replicate measurements.

^dCV = SD/$\left[\overline{X}\right]$ × 100%.

^eRE = ($\left[\overline{X}\right]$ − [X_N])/[X_N] × 100%.