Table 3.

Intra- and inter-assay accuracy and precision of DHT, α-diol, and β-diol in pooled mouse sera (n = 5).

Intra-Assaya
	DHT			α-diol			β-diol
[XN] (ng/ml)	$\left[\overline{X}\right]$ ± SD (ng/ml)	CV (%)	RE (%)	$\left[\overline{X}\right]$ ± SD (ng/ml)	CV (%)	RE (%)	$\left[\overline{X}\right]$ ± SD (ng/ml)	CV (%)	RE (%)
0.150	0.14 ± 0.01	7	−7	0.15 ± 0.01	7	0	0.15 ± 0.01	7	0
2.50	2.30 ± 0.02	1	−8	2.43 ± 0.03	1	−3	2.51 ± 0.07	3	0
40.0	37 ± 1	3	−8	38 ± 2	5	−5	39 ± 1	3	−3

Inter-Assayb
	DHT			α-diol			β-diol
[XN] (ng/ml)	$\left[\overline{X}\right]$ ± SD (ng/ml)	CV (%)	RE (%)	$\left[\overline{X}\right]$ ± SD (ng/ml)	CV (%)	RE (%)	$\left[\overline{X}\right]$ ± SD (ng/ml)	CV (%)	RE (%)
0.150	0.14 ± 0.01	7	−7	0.15 ± 0.01	7	0	0.16 ± 0.01	6	7
2.50	2.4 ± 0.1	4	−4	2.5 ± 0.1	4	0	2.47 ± 0.05	2	−1
40.0	36.7 ± 0.4	1	−8	38.6 ± 0.5	1	−4	39.8 ± 0.7	2	−1

^aMeasured by five replicate measurements of each QC sample within a validation batch.

^bMeasured by five parallel measurements of five identical QC samples at each concentration over five validation batches.