. Author manuscript; available in PMC: 2019 Jan 9.

Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2018 May 16;1090:22–35. doi: 10.1016/j.jchromb.2018.05.008

Table 4.

Recovery of DHT, α-diol, and β-diol in pooled mouse sera (n = 5).

[X_N] (ng/ml)	DHT			α-diol			β-diol
	Recovery_x^a- ± SD (%)	Recovery_{IS −} ^b ± SD (%)	IS Normalized Recovery^c - ± SD (%)	Recovery_x - ± SD (%)	Recovery_{IS -} ± SD (%)	IS Normalized Recovery -± SD (%)	Recovery_{x -} ± SD (%)	Recovery_{IS -} ± SD (%)	IS Normalized Recover-y ± SD (%)
0.150	90 ± 4	92 ± 6	98 ± 4	106 ± 3	104 ± 5	102 ± 3	89 ± 6	90 ± 3	99 ± 3
2.50	89 ± 3	86 ± 5	103 ± 3	108 ± 5	105 ± 3	103 ± 8	92 ± 5	93 ± 2	99 ± 6
40.0	91 ± 5	90 ± 3	101 ± 5	102 ± 6	100 ± 2	102 ± 2	95 ± 3	95 ± 4	100 ± 4

Recovery_X = (mean peak area value of spiked analyte in steroid-free sera matrix)/(mean peak area value of spiked analyte in extracted steroid-free sera matrix) × 100%.

Recovery_IS = (mean peak area value of spiked IS in steroid-free sera matrix)/(mean peak area value of spiked IS in extracted steroid-free sera matrix) × 100%.

IS Normalized Recovery = (Recovery_X)/(Recovery_IS) × 100%.