. Author manuscript; available in PMC: 2019 Jan 9.

Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2018 May 16;1090:22–35. doi: 10.1016/j.jchromb.2018.05.008

Table 5.

Matrix factors of DHT, α-diol, and β-diol in six individual lots of mouse sera (n = 5).

Sera	[X_N] (ng/ml)	DHT			α-diol			β-diol
		MF_x^a ± SD	MF_IS^b ± SD	IS normalized MF^c ± SD	MF_X ± SD	MF_IS ± SD	IS normalized MF ± SD	MF_X ± SD	MF_IS ± SD	IS normalized MF ± SD
Lot 1	0.150	0.99 ± 0.02	0.96 ± 0.01	1.03 ± 0.02	0.99 ± 0.06	1.00 ± 0.01	0.99 ± 0.05	1.00 ± 0.02	0.98 ± 0.01	1.02 ± 0.03
	2.50	0.98 ± 0.01	0.97 ± 0.02	1.01 ± 0.03	0.93 ± 0.05	0.95 ± 0.01	0.98 ± 0.05	0.99 ± 0.02	0.97 ± 0.01	1.02 ± 0.03
	40.0	1.02 ± 0.01	0.99 ± 0.04	1.03 ± 0.05	0.99 ± 0.03	0.98 ± 0.02	1.01 ± 0.05	1.01 ± 0.03	0.98 ± 0.04	1.03 ± 0.04
Lot 2	0.150	1.03 ± 0.02	1.03 ± 0.03	1.00 ± 0.02	1.01 ± 0.04	0.99 ± 0.01	1.02 ± 0.04	0.97 ± 0.03	0.97 ± 0.02	1.00 ± 0.02
	2.50	1.01 ± 0.03	1.01 ± 0.01	1.00 ± 0.03	0.99 ± 0.03	0.96 ± 0.04	1.03 ± 0.04	0.95 ± 0.05	0.96 ± 0.02	0.99 ± 0.03
	40.0	0.99 ± 0.02	0.98 ± 0.03	1.01 ± 0.03	0.98 ± 0.04	1.00 ± 0.02	0.98 ± 0.06	0.98 ± 0.02	0.97 ± 0.03	1.01 ± 0.03
Lot 3	0.150	0.97 ± 0.04	0.98 ± 0.02	0.99 ± 0.06	0.96 ± 0.03	0.99 ± 0.03	0.97 ± 0.06	0.99 ± 0.05	0.98 ± 0.01	1.01 ± 0.05
	2.50	1.04 ± 0.01	0.99 ± 0.06	1.05 ± 0.01	1.04 ± 0.02	1.01 ± 0.03	1.03 ± 0.03	1.03 ± 0.02	1.01 ± 0.05	1.02 ± 0.02
	40.0	0.95 ± 0.03	0.94 ± 0.02	1.01 ± 0.02	0.98 ± 0.04	0.99 ± 0.02	0.99 ± 0.04	1.06 ± 0.01	1.00 ± 0.03	1.06 ± 0.04
Lot 4	0.150	0.97 ± 0.04	0.99 ± 0.01	0.98 ± 0.05	0.96 ± 0.03	0.98 ± 0.02	0.98 ± 0.05	1.02 ± 0.03	1.04 ± 0.02	0.98 ± 0.03
	2.50	0.99 ± 0.02	0.98 ± 0.03	1.01 ± 0.05	1.02 ± 0.01	1.01 ± 0.04	1.01 ± 0.05	1.04 ± 0.02	1.02 ± 0.03	1.02 ± 0.02
	40.0	1.02 ± 0.04	1.01 ± 0.02	1.01 ± 0.02	1.04 ± 0.03	1.02 ± 0.01	1.02 ± 0.03	0.99 ± 0.03	1.01 ± 0.04	0.98 ± 0.03
Lot 5	0.150	0.95 ± 0.03	0.98 ± 0.02	0.97 ± 0.05	0.97 ± 0.02	0.99 ± 0.04	0.98 ± 0.04	0.97 ± 0.04	0.98 ± 0.03	0.99 ± 0.03
	2.50	0.98 ± 0.04	0.99 ± 0.02	0.99 ± 0.04	0.99 ± 0.04	1.01 ± 0.03	0.98 ± 0.04	1.05 ± 0.04	1.03 ± 0.03	1.02 ± 0.03
	40.0	1.02 ± 0.03	1.01 ± 0.04	1.01 ± 0.04	1.05 ± 0.01	1.03 ± 0.04	1.02 ± 0.05	1.00 ± 0.03	0.99 ± 0.04	1.01 ± 0.03
Lot 6	0.150	1.01 ± 0.02	0.99 ± 0.03	1.02 ± 0.05	0.99 ± 0.06	0.94 ± 0.03	1.05 ± 0.02	0.99 ± 0.05	1.00 ± 0.03	0.99 ± 0.05
	2.50	0.99 ± 0.02	0.95 ± 0.03	1.04 ± 0.03	0.98 ± 0.03	0.97 ± 0.01	1.01 ± 0.04	0.97 ± 0.04	0.98 ± 0.02	0.99 ± 0.06
	40.0	0.98 ± 0.04	1.00 ± 0.04	0.98 ± 0.04	1.03 ± 0.05	0.99 ± 0.02	1.04 ± 0.05	0.99 ± 0.05	0.98 ± 0.03	1.01 ± 0.03

MF_X = (mean peak area value of spiked analyte in extracted steroid-free sera matrix)/(mean peak area value of spiked analyte in the mobile phase).

MF_IS = (mean peak area of spiked IS in the extracted steroid-free sera matrix)/(mean peak area of spiked IS in the mobile phase).

IS normalized MF = MF_X/MF_IS.