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. 2019 Jan 9;14(1):e0209435. doi: 10.1371/journal.pone.0209435

Fig 7. MBCD decreased stemness in MDA-MB-231.

Fig 7

(A) Colony formation assay was performed. MDA-MB-231 cells were plated at low density (1000 cells/well) in 6 well plates. After 24 hr, cells were treated with MBCD (1mM). After 5 days, formed colonies were stained with crystal violet. Photos of the wells were taken by a camera (Upper panel of A). Colonies were visualized by inverted bright field microscope. Arrows depict the colonies. (B) Bars represent the average colony count of three independent fields. Value represents mean ± SEM, **p < .01 vs. control. (C) Soft agar assay of MDA-MB-231 cells in presence and absence of MBCD (1mM). Day zero and day 9 pictures were shown in the figure. Arrows depict the spheres. (D) Bars showed average of spheroid counts. Value represents mean ± SEM of three independent measurements, **p < .01 vs. control. (E) RT-PCR analysis was performed using total RNA and gene specific primers. Decreased levels of stemness markers CD44 and BMI1 were found in MBCD treated cells. GAPDH was measured as loading control. Densitometry analysis was shown in S1C Fig.