Skip to main content
. 2016 Aug 23;138(36):11996–12002. doi: 10.1021/jacs.6b07977

Figure 1.

Figure 1

Rate of primer extension increases or decreases with time, depending on the pH of the monomer stock solution. (a) The primer extension assay measures the rate at which the primer in an RNA primer template duplex is converted to longer extension products by nonenzymatic reaction with 2-MeImpN monomers (1). (b) Using a 2-MeImpG stock at pH 9.88, the kobs of primer extension increases over time. (c) Using a 2-MeImpG stock at pH 7.79, the kobs of primer extension decreases over time. Error bars indicate ± one SD.