Figure 2.

Effects on the potency of DkTx for TRPV1 activation upon alteration of protein–protein and protein–lipid interfaces. (a) Dose–response plots of the variants of exclusively channel-interacting (top) and lipid-interacting (bottom) residues of DkTx. The K1 knot variants of DkTx are depicted on the left and K2 variants on the right. (b) EC₅₀ values of all DkTx variants. The bars in green correspond to variants of exclusively channel-interacting DkTx residues, and the ones in blue are of those that interact with lipids. The EC₅₀ values for the W53A and W53L variants could not be obtained owing to their extremely low potency (this is denoted by the arrows on the top of the bars corresponding to these variants). “WT” is an abbreviation for “wild-type”. Each data point is an average of three to five recordings, and the error bars correspond to standard deviation values.