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. 2018 Dec 7;10(12):3683–3701. doi: 10.18632/aging.101662

Figure 2.

Figure 2

Enhanced canonical and non-canonical TGF-β signaling in SMAD4-interfered HASMCs. (A) Real-time PCR assay for the expression of canonical and non-canonical TGF-β signaling molecules in different HASMCs. (B) Western blot assay for the expression of canonical and non-canonical TGF-β signaling mediators in HASMCs transfected with siRNA-NC or siRNA-SMAD4. (C) Western blot assay for the expression of canonical and non-canonical TGF-β signaling mediators in HASMCs carrying rs12455792 CC, CT or TT genotypes. (D, E) Quantitative analysis for B and C, respectively. (F) Real-time PCR assay for the expression of essential chemotactic factors in different HASMCs. The experiments were repeated three times. Data were presented as mean ± SD. *P<0.05, **P<0.01, ns: not significant.