Table 1. Strains and plasmids used in this study.
Yeast Strain | Genotype |
BY4741 | MATa his3Δ1 lys2Δ0 met15Δ0 ura3Δ0 | ||
---|---|---|---|
BY4741 pdr5Δ | MATa his3D1 leu2D0 met15D0 ura3D0 pdr5::kanMX4 | ||
BY4741 ump1Δ | MATa his3D1 leu2D0 met15D0 ura3D0 ump1::kanMX4 | ||
BY4741 rpn4Δ | MATa his3D1 leu2D0 met15D0 ura3D0 rpn4::kanMX4 | ||
BY4741 cue5Δ | MATa his3D1 leu2D0 met15D0 ura3D0 cue5::kanMX4 | ||
Plasmids | Type of Plasmid | Source | |
pYX222-SNCA | 2µ | [34] | |
pRS416-GFPAtg8 | 2µ | [21] | |
pYES2-Ub-G76V-GFP | 2µ | Addgene |