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. 2018 Dec 12;10(12):3794–3805. doi: 10.18632/aging.101673

Figure 2.

Figure 2

SEVO impairs neuron cell proliferation in vitro likely through decreasing CCNA2. Identical number of HCV-2 neural cells was exposed to different doses of SEVO for 48 hours and then subjected to analysis. (A-B) Ki-67+ staining on HCN-2 cells, shown by representative images (A), and by quantification (B). (C) The total cell number based on DAPI staining. (D) Western blotting for CDKs and cyclins in 5% SEVO-exposed HCN-2 cells vs gas-exposed HCN-2 cells. (E) Quantification of changes in CCNA2 protein. (F) RT-qPCR for CCNA2 mRNA. *p<0.05. NS: non-significant. N=5. Scale bars are 50µm.