Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Mus musculus) |
Pcdh1 | this paper | NCBI: NM_029357.3 | cloned from isolated RNA from mouse olfactory epithelium |
| Gene (Mus musculus) |
Pcdh7 | this paper | NCBI: NM_001122758.2 | cloned from isolated RNA from mouse olfactory epithelium |
| Gene (Mus musculus) |
Pcdh8 | this paper | NCBI: NM_001042726.3 | cloned from isolated RNA from mouse olfactory epithelium |
| Gene (Mus musculus) |
Pcdh9 | this paper | NCBI: NM_001271798.1 | cloned from isolated RNA from mouse olfactory epithelium |
| Gene (Mus musculus) |
Pcdh10 | this paper | NCBI: NM_001098172.1 | cloned from isolated RNA from mouse olfactory epithelium |
| Gene (Mus musculus) |
Pcdh11x | this paper | NCBI: NM_001271809.1 | cloned from isolated RNA from mouse olfactory epithelium |
| Gene (Mus musculus) |
Pcdh17 | this paper | NCBI: NM_001013753.2 | cloned from isolated RNA from mouse olfactory epithelium |
| Gene (Mus musculus) |
Pcdh18 | this paper | NCBI: NM_130448.3 | cloned from isolated RNA from mouse olfactory epithelium |
| Gene (Mus musculus) |
Pcdh19 | this paper | NCBI: NM_001105246.1 | cloned from isolated RNA from mouse olfactory epithelium |
| Gene (Mus musculus) |
Pcdhb11 | this paper | NCBI: NM_053136.3 | cloned from isolated RNA from mouse olfactory epithelium |
| Strain, strain background (Mus musculus) |
FVB/NJ | The Jackson Laboratory | 1800 | |
| Strain, strain background (Mus musculus) |
C57BL/6J | The Jackson Laboratory | 664 | |
| Strain, strain background (Mus musculus) |
CD-1 | Charles River | 22 | |
| Cell line (Homo sapiens) |
K-562 | ATCC | CCL-243 | |
| Biological sample (Mus musculus) |
primary olfactory sensory neurons |
this paper | isolated for single cell analysis from P6-P8 mice, both sexes |
|
| Biological sample (Mus musculus) |
olfactory epithelium |
this paper | isolated and sectioned for RNA in situ hybridization, at ages indicated in paper, both sexes |
|
| Antibody | moust anti-GFP | Thermo Scientific | MA5-15256 | 1:4000 |
| Antibody | mouse anti-RFP | Thermo Scientific | MA5-15257 | 1:2000 |
| Antibody | mouse anti-FLAG | Thermo Scientific | MA1-91878 | 1:6000 |
| Antibody | mouse anti-Transferrin Receptor (TfR) |
Thermo Scientific | 13–6800 | 1:1000 |
| Recombinant DNA reagent |
N1-p2a- GFP or RFP |
this paper | modified from Clontech N1-eGFP |
|
| Recombinant DNA reagent |
N1-GFP or RFP | this paper | modified from Clontech N1-eGFP |
|
| Sequence- based reagent |
this paper | see supplemental tables for all primers |
||
| Commercial assay or kit |
Pierce Cell Surface Isolation Kit |
Thermo Scientific |
89881 | |
| Commercial assay or kit |
Ingenio Electroporation Kit |
Mirus | MIR 50118 | |
| Chemical compound, drug |
Valproic acid sodium salt |
Sigma-Aldrich | P4543 | 4 μM |
| Software, algorithm |
Co-Ag index | this paper | code written in Mathematica (Wolfram Research) |
|
| Software, algorithm |
Aggregate size measurement |
this paper | code written in Mathematica (Wolfram Research) |
|
| Software, algorithm |
Cell aggregation Monte Carlo Simulator |
this paper |
https://github.com/shazanfar/cellAggregator
; Ghazanfar et al., 2016 |