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. 2018 Oct 13;47(1):152–167. doi: 10.1093/nar/gky947

Figure 3.

Figure 3.

shRNA-induced Dnmt3l KD does not lead to gain of methylation. (A) Western blot analysis showing that the three Dnmt3l shRNAs induced DNMT3L depletion with varying efficiencies (#3>#2>#1). The experiment was performed twice. (B) Southern blot analysis showing that Dnmt3l KD led to slight decreases of methylation at the major satellite repeats. (C) Bisulfite sequencing analysis of the same five gene regions described in Figure 2F, which showed either loss of or no changes in methylation in Dnmt3l KD mESCs. Control (shLuc) and Dnmt3l KD (shDnmt3l #3) DNA samples from experiment 1 were used for this experiment.