Table 3.
Chromosome | Start position | End position | Strand |
---|---|---|---|
1. Chromosome I | –1 | 0 | – |
2. Chromosome II | 813184 | 813185 | + |
Coordinates were calculated based on (1.) one read that aligned to the 5′-most end of the forward strand of chromosome I (zero-based start/end positions should be 0/1) and (2.) another read that aligned to the 5′-most end of the reverse strand of chromosome II (zero-based start/end positions should be 813183/813184) of the Saccharomyces cerevisiae genome. To prevent errors in downstream analyses, emRiboSeqProcessor should have screened the calculated coordinates for biological relevance prior to saving them to the output BED file.