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. 2018 Nov 12;47(1):253–265. doi: 10.1093/nar/gky1125

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — BREX+ cells extracts lack nuclease activity under conditions when restriction-endonuclease activity is detected. Cell extracts prepared from Escherichia coli cells harboring a plasmid containing EcoRV restriction-modification system genes (lane 2), pBREXAL plasmid (lane 3) or pBTB-2 control vector (lane 4) were combined with unmodified λ phage DNA, incubated and reaction products were resolved by agarose gel electrophoresis. Lane 1 is a control lane, phage genome incubated with buffer. M is a molecular weight marker.