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. 2018 Sep 12;47(1):e1. doi: 10.1093/nar/gky820

Figure 4.

Figure 4.

Quantification of gene copy number using CRISPR-Cap. We prepared genomic DNA samples containing three different copy numbers of the bla gene from (i) E. coli EcHB3, (ii) EcHB3 containing pBR322 (middle copy), (iii) and EcHB3 containing pUC19 (high copy). (A) E. coli EcHB3 has a single copy of bla in its genome. E. coli EcHB3 genomic DNA with either pBR322 (B) or pUC19 (C) contains middle and high copy numbers of the bla gene, respectively. Dashed lines represent the average sequencing depth of the bla gene region in each product. The average sequencing depth of the bla gene gradually increased according to the copy number.