Figure 6.

The tryptophan residue in the loop of the RABV PRNTase domain is essential for terminal de novo initiation, but not for internal initiation. WT and mutant RABV L proteins were subjected to transcription with ATP, [α-³²P]CTP, P and the indicated templates (see Figure 5B). Amounts of [α-³²P]CMP incorporated into AC and AACA (marked by closed and open arrowheads, respectively) are shown in the graphs. The dot-plots, columns, and error bars represent the individual values, means, and standard deviations, respectively (n = 3).