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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: Biol Psychiatry. 2018 Aug 17;85(3):237–247. doi: 10.1016/j.biopsych.2018.07.027

Figure 3. Chemogenetic stimulation of the LC or LC projections to the vPAG promotes wakefulness.

Figure 3.

A) AAV-hSyn-DIO-hM3D(Gq)-mCherry was injected into the LC of TH-Cre+ mice. Scale bars = 100 μm. B-C) CNO (1 mg/kg)-induced activation of Gq DREADDs in the LC significantly increased sleep latency (n = 8; *p < 0.05). D-E) Site-specific infusion of CNO (1 μg/0.3 μL) into the vPAG to stimulate LC projections to the vPAG similarly increased sleep latency (n=8; *p < 0.05).