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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: FEBS J. 2018 Dec 1;286(1):151–168. doi: 10.1111/febs.14702

Figure 1.

Figure 1.

A. Secondary structure predictions for HCRLC and mutants. Modeled structures of human ventricular RLC (MYL2) WT, R58Q and S15D-R58Q, using I-TASSER. Note that the distance between the C-α of R58 and S15 is changed in HCM-R58Q mutant and that the phosphomimic variant reverses this value to the level of WT. B. Effect of the R58Q mutation and S15D-R58Q phosphomimic on the CD spectra of human cardiac RLC. Far-UV CD was performed utilizing a 1-mm path quartz cell in a Jasco J-720 spectropolarimeter. Spectra were recorded at 190–250 nm with a bandwidth of 1 nm. [θ]MRE at 222 nm was used to calculate the α-helical content (fH) using the equation: [θ]222 = −30,300 fH − 2,340.