Table 1. Efficiency of RLC depletion and mutant RLC reconstitution in porcine cardiac myosin and porcine cardiac papillary muscles (PM).
Depleted | WT | R58Q | S15D-R58Q | |
---|---|---|---|---|
Porcine Cardiac Myosin | ||||
% RLC Present | 19.61 ± 1.78 | 87.20 ± 6.34 | 75.74 ± 9.00 | 86.88 ± 4.25 |
n= # SDS-PAGE | 7 | 4 | 5 | 5 |
Porcine Cardiac papillary muscles | ||||
% RLC Present | 16.89 ± 1.17 | 61.31 ± 2.48 | 54.00 ± 4.02 | 64.50 ± 3.17 |
n= # fibers | 9 | 13 | 13 | 12 |