. Author manuscript; available in PMC: 2020 Jan 1.

Published in final edited form as: FEBS J. 2018 Dec 1;286(1):151–168. doi: 10.1111/febs.14702

Table 2. Steady-state rigor (no ATP) binding of RLC depleted/reconstituted myosin to pyrene-labeled F-actin and actin-activated myosin ATPase activity.

Values are means ± SEM for n = # independent experiments.

Parameter	WT	R58Q	S15D-R58Q
Parameter	Steady-state rigor binding
Kd (nM)	2.13 ± 0.56	13.26 ± 1.45^****	3.53 ± 0.66
n	6	4	4
	*ATPase activity*
Vmax (s⁻¹)	0.32 ± 0.01	0.18 ± 0.02 ^****	0.36 ± 0.01
Km (μM)	2.56 ± 0.24	4.05 ± 0.65	3.72 ± 0.46
n	12	17	13

^****

p<0.0001 versus WT, S15D-R58Q, one-way ANOVA with Tukey’s multiple comparison test.