Table 3. Actin-myosin dissociation rates (k_obs in s⁻¹).

Values are means ± SEM for n = # independent titrations of actin-myosin complex with [Mg-ATP]; 7–13 transients were averaged for each run.

System	[MgATP]
	10 μM	25 μM	40 μM	80 μM	150 μM	250 μM
WT	8.25±0.44 n=19	15.65±0.96 n=11	27.27±2.03 n=18	48.89±2.57 n=21	82.18±3.78 n=18	106.63±7.76 n=13
S15D-R58Q	7.29±0.43 n=19	14.69±0.89 n=16	25.30±1.31 n=23	41.52±1.24 n=22	73.99±1.76 n=35	110.65±4.67 n=33
R58Q	7.26±0.31 n=12	15.13±0.67 n=10	23.61±1.05 n=20	45.64±2.58 n=28	69.19±2.85** n=27	107.11±4.50 n=31

^**p<0.01 versus WT, one-way ANOVA with Tukey’s multiple comparison test.