Fig. 2.

Parameters used to assess harmonization between study sites. (A) Time of day when blood samples were drawn. UCLA uses a standard time (i.e., midday) to collect all blood samples. More variation was observed at UEF and Melbourne. (B) Anesthesia duration during blood sampling. (C) Plasma volume obtained per draw at each study sites. Overall, 6% of samples (n = 112/1784) collected so far have less than 200 µL of stored plasma. The number of samples falling in that category varied between sites as it was 9% of all samples in UEF, 0.5% in Melbourne, and 6% in UCLA. (D-F) Learning effect in the plasma volumes collected at each site (D, UEF; E, Melbourne; F, UCLA). All sites showed improvement in obtaining > 200 µL of plasma in later cohorts. Abbreviations: SD, standard deviation; UEF, University of Eastern Finland; UCLA, University of California, Los Angeles.