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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: Neuropeptides. 2018 Nov 30;73:78–88. doi: 10.1016/j.npep.2018.11.009

Table 1.

PCR sequences for genotyping

Primer pair Sequence Product size
Lox p site
NPYgtloxpF
NPYgtloxpR

GCCTCCGTGCCTCTTATCTT
GATTTTGGAGGCAACAGAGC

WT – 204 bp
Mut – 270 bp
Frt site
NPYgtfrtF
NPYgtfrtR

CCAATCCTATCGCATCCCTA
CTATCTGGAGGTGGCAGGTC

WT – 198 bp
Mut – 278 bp
NPY deletion
NPYgtloxpF
NPYgtfrtF
NPYgtfrtR

GCCTCCGTGCCTCTTATCTT
CCAATCCTATCGCATCCCTA
CTATCTGGAGGTGGCAGGTC

WT – 198 bp
KO – 353 bp