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. Author manuscript; available in PMC: 2020 Jan 15.
Published in final edited form as: Acta Biomater. 2018 Dec 1;84:169–179. doi: 10.1016/j.actbio.2018.11.030

Fig. 3.

Fig. 3.

DNA synthesis by GFP+ and GFP− cells is influenced by %HA and type of peptide. Overall DNA synthesis by GFP+ cells was greater than that by the GFP− cells. (A) GFP+ cells showed the highest DNA synthesis on the 2% HA hydrogels functionalized with RGD. Two-way ANOVA revealed significance for peptide (p < 0.05) and gel percentage (p < 0.0001). (B) Two-way ANOVA revealed significance for peptide (p < 0.05) and gel percentage (p < 0.0001) on EdU incorporation by GFP− cells. Tukey’s post hoc test showed a significant increase in DNA synthesis on the 3% peptide-functionalized hydrogels compared with 1% HA. Cells were cultured in vitro for 24 h and the percentage of cells that synthesized new DNA was determined by labeling for EdU incorporation (*p < 0.05; **p < 0.005). Error bars = SD; n ≥ 360 cells per hydrogel combination. Three independent experiments were averaged with n ≥ 3 technical replicates per experiment.