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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: Hypertension. 2019 Feb;73(2):390–398. doi: 10.1161/HYPERTENSIONAHA.118.12084

Figure 1. Induction of GRP78 by pressure overload in the heart.

Figure 1.

A. GRP78 protein expression was increased in the heart in response to pressure overload. Thoracic aortic constriction (TAC) was performed to induce cardiac hypertrophy. Note that multiple protein makers of cardiac hypertrophy show strong upregulation. GAPDH was used as a loading control. B. Quantification of A supported significant elevation of GRP78 by pressure overload. N = 6. C. GRP78 was upregulated in cardiac myocytes by hypertrophy stimulation. Cultured neonatal rat ventricular myocytes (NRVMs) were treated with phenylephrine (50 μM) for 24 hours. GRP78 was detected by western blotting. PE treatment stimulated cellular hypertrophic growth as indicated by increases in βMHC, Rcan1.4, and Anf. D. Quantification of C showed significant upregulation of GRP78. N = 5. *, p < 0.05; **, p < 0.01.