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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: Cerebellum. 2019 Feb;18(1):99–108. doi: 10.1007/s12311-018-0961-2

Fig 3. Co-localization of DCL64 liposomes with LDLr family.

Fig 3.

(A) DCL64 liposomes incorporated with oligonucleotides (Cy3) co-localized with LDLr and LRP-1 (Alexa 488 green), but not with caveolin or SR-B1 (Alexa 488 green). (Top left) BMECs only; (top right) BMECs incubated with DCL64; (middle) BMECs incubated with DCL64 followed by antibodies specific for LDLr (left) or LRP-1 (right); (bottom) BMECs incubated with DCL64 followed by antibodies specific for caveolin (left) or SR-B1 (right). (B) LDLr and LRP-1 blocking peptides decreased the binding of fluorescent DCL64 liposomes to BMECs. BMECs were preincubated with LDLr or LRP-1 blocking peptides before incubated with DCL64; (left) 0 µg blocking peptides; (center) 0.1 µg blocking peptides; (right) 1.0 µg blocking peptides. Scale bars: (Panel A) — = 10 microns; (Panel B) ――― = 20 microns.