Table 1. Characteristics of RFLP-PCR primers, restriction endonucleases and product size.
Polymorphic positions | Primer sequence | Restriction enzyme | Product size (bp) |
|
Wild-type | Minore-type | |||
TNF-а-863 | 5′ GGCTCTGAGGAATGGGTTAC 3′ | BstBAI | 125 | 102; 23 |
C→A | 5′ CTACATGGCCCTGTCTTCGTTACG 3′ | |||
TNF-а-308 | 5′ AGGCAATAGGTTTTGAGGGCCAT 3′ | Bsp19I | 97; 20 | 118 |
G→A | 5′ ACACTCCCCATCCTCCCGGCT 3′ | |||
TNF-а-238 | 5′ AGAAGACCCCCCTCGGAACC 3′ | Msp I | 132; 20 | 152 |
G→A | 5′ ATCTGGAGGAAGCGGTAGTG 3′ |