Table 1. Examples of experimental features from somatic cell to iPSCs.
| Cell source | Culture condition | Reprogramming factors | Delivery method | Time length (d) | Efficiency (%) | Reference |
| Mouse fibroblasts | Cell feeder | Oct3/4, Sox2, C-myc, Klf4 | Retrovirus | N/A | 0.02 | [9] |
| Human fibroblasts | Cell feeder | Oct4, Sox2, Nanog, Lin28 | Lentivirus | 15 | 0.022 | [22] |
| Human fibroblasts | Cell feeder | Oct3/4, Sox2, Klf4, Sall4 | Retrovirus | 25 | 0.014 | [23] |
| Human blood mononuclear cells | Cell feeder | Oct4, Sox2, Klf4, C-myc, Lin28 | Plasmid | 14 | 0.05 | [11] |
| Human fibroblasts | Cell feeder | Oct3/4, Sox2, Klf4, Glis1 | Retrovirus | 22 | 0.17 | [24] |
| Human fibroblasts | Cell feeder | Oct3/4, Sox2, Klf4, L-myc, Lin28, P53 | Plasmid, shRNA | 14 | 0.03 | [25] |
| Human keratinocytes | Cell feeder | Oct4, Sox2, Klf4, C-myc, Rem2 | Retrovirus | 12 | 0.2 | [26] |
| Human fibroblasts | Cell feeder | Oct4, Sox2, Myc, Klf4, Sv40 large T antigen | Lentivirus | 14 | 0.46 | [27] |
| Human fibroblasts | Cell feeder | Oct3/4, Sox2, C-myc, Klf4 | Adenovirus | 25 | 0.0002 | [28] |
| Mouse and human fibroblasts | Cell feeder | Oct3/4, Sox2, C-myc, Klf4 | 2A peptide | 9 | 2.5 | [13] |
| Human fibroblasts | Cell feeder | Oct3/4, Sox2, C-myc, Klf4 | PiggyBac transposon | 14 | 0.01 | [13] |
| Human fibroblasts | Cell feeder | Oct3/4, Sox2, C-myc, Klf4 | PiggyBac transposon | 10-14 | 0.03 | [29] |
| Human adipose stromal cells | Cell feeder | Oct4, Sox2, Nanog, Lin28 | Minicircle DNA | 18 | 0.0075 | [30] |
| Human fibroblasts | Xeno-free, feeder-free | Oct3/4, Sox2, Klf4, L-myc, Lin28, TP53, A-83-01, sodium butyrate, CHIR99021 | Plasmids, shRNA, small molecules | 16 | 0.1 | [10] |
| Mouse fibroblasts | Cell feeder | Mir-200c, Mir-302s, Mir-369s | MiRNA | 15 | 0.1 | [12] |
| Human fibroblasts | Cell feeder | Mir-200c, Mir-302s, Mir-369s | MiRNA | 20 | 0.002 | [12] |
| Human fibroblasts | Cell feeder | Oct4, Sox2, VPA | Retrovirus, small molecules | N/A | 0.01 | [31] |
| Human fibroblasts | Cell feeder | Oct4, Sox2, Klf4, VPA | Retrovirus, small molecules | N/A | 1 | [31] |
| Human bone mesenchymal stromal cell | Feeder-free | Oct4, Sox2, Nanog, Lin28, Sv40lt, Klf4, C-myc, A-83-01, PD325901, CHIR99021, HA-100 | Plasmids, small molecules | N/A | N/A | [18] |
| Mouse fibroblasts | Cell feeder | Oct4, tranylcypromine, VPA, CHIR99021, 616452 | Lentivirus, small molecules | 18d | 0.03 | [32] |
| Human fibroblasts | Xeno-free, feeder-free | Oct3/4, Sox2, C-myc, Klf4 | Lentivirus | 7-14d | 0.3 | [33] |
iPSCs: Induced pluripotent stem cells; VPA: Valproic acid.