Fig. 2.

Alteration in specific macrophage subset in the operated conjunctiva treated with VPA. Cells isolated from day 2 operated tissues, treated with either PBS (control) or VPA, were first gated as shown in panel 1 based on SSC vs FSC, followed by gating for live cells using 7-AAD (not shown). Panel 2 shows scatter from live cells stained with negative control IgG2b antibodies. Panel 3 shows positive staining for both CD45 and F4/80 antibodies, with gates representing CD45^highF4/80^low (smaller oval) or CD45^lowF4/80^high (larger oval). Panel 4 shows scatter derived from the gate indicated by the long horizontal arrow in panel 3. Panel 5 shows scatter from live cells that label positive for both CD45 and CD11b antibodies. a Gating for all cells to reveal CD45^highF4/80^low or CD45^lowF4/80^high subsets. b Gating for smaller FSC cells to reveal mainly the CD45^lowF4/80^high subset. c Gating for larger FSC cells to reveal mainly the CD45^highF4/80^low subset. d Five independent sets of experiments, with each set of experiment consisting of either PBS or VPA treatment, were analyzed for the CD45^highF4/80^low subset based on the gating shown in c. The fold difference in the % live CD45^highF4/80^low subset comparing VPA and PBS treatment is indicated. *p < 0.05