Fig. 1.

TRPM4 blockade improves human cerebral microvascular endothelial cell (HCMEC) survival and migration after hypoxia reoxygenation. a HCMECs were incubated in a hypoxia chamber for 5 h followed by reoxygenation and re-glucose for 12 h. Scratch wound healing assay was performed to evaluate cell growth. TRPM4 was blocked by 5 μM 9-phenanthrol (9-phe). Control cells were treated with DMSO. Scale bars 200 μm. b The extent of gap reduction in cells with 9-phe treatment was quantified and compared to control cells (n = 4, * p = 0.0236, Student’s t test). c Summary of cell counting (n = 4, *p = 0.0205, Student’s t test)