Fig. 4.

TRPM4 inhibition reduces infarction and improves motor functions. a Immunofluorescent staining of TRPM4 and vWF in rats receiving scrambled (Scram) or TRPM4 siRNA (siRNA) treatment. In vivo siRNA was delivered intravenously prior operation. Stroke reperfusion was achieved by 2-h MCAO and 24-h recanalization. Scale bars 50 μm. b Western blot was performed to detect TRPM4 expression in the ipsilateral hemispheres. Sham-operated hemisphere served as the control brain. c TTC staining of rat brains receiving scrambled or TRPM4 siRNA treatment. d Summary of the total infarct volume corrected for edema (n_siRNA = 6, n_scram = 5, *p = 0.0271, Student’s t test). e Comparison of the infarct volumes in the cortex and striatum (n_siRNA = 6, n_scram = 5, *p = 0.0476, Student’s t test). f Section-by-section infarct area distribution (n_siRNA = 6, n_scram = 5, *p = 0.0492; **p = 0.0083, Student’s t test). g Assessment of motor functions by Rotarod test (n = 10 per group, *p < 0.05, siRNA vs scram, repeated ANOVA with Bonferroni’s post hoc analysis)