Fig. 6.

Treatment with UroA/UAS03 mitigate DSS-induced chronic colitis. a C57BL/6 mice (7–8 week age old) were treated with four cycles of DSS (2%) with 7 days/cycle with an interval of 14 days with regular water. Control group of mice (n = 5) received the regular water without DSS. UroA/UAS03 (20 mg/kg/day/body weight) that was resuspended in 0.25% sodium carboxymethylcellulose (CMC) solution (n = 9) or vehicle (CMC) (n = 9) was administered on 4th and 6th day of each DSS cycle and one treatment while on regular water. n = 5/control; n = 9/veh and UroA; n = 8/UAS03 group) Mice were euthanized at day 89 and the colitis phenotype was characterized. b Intestinal permeability using FITC-dextran was evaluated. c Representative colon images d colon lengths, e ratios of colon weight/length are shown. f Serum levels of IL-6, IL-1β, and TNF-α were measured using ELISA methods. g MPO levels were determined in colon tissues. h Cldn4 expression in the colons of these mice (n = 3) was measured by immunoblots. Statistics performed using unpaired t-test using Graphpad Prism software. Error bars, ±SEM ***p < 0.001; **p < 0.01; *p < 0.05. Source Data are provided as a Source Data File