Fig. 2.

EBNA2 expression decreases miR-34a in transfected B lymphoma cells by affecting its transcription. miR-34a (top panels) and pre-miR-34a expression (middle panels) was verified by real-time qPCR in (a) U2932 DLBCL transfected with EBNA2 and (b) BL41K3 are transfected with estrogen-inducible EBNA2. miR-34a promoter carrying Luc reporter activity was analyzed in U2932 and BL41 and their EBNA2-expressing derivatives (lower panels). The figure shows standard deviation (SD) of the average of three different experiments. Each experiment was performed in biological triplicates for each sample and repeated three times. p values were calculated with unpaired t test. In all cell lines miR-34a expression (****) p < 0.0001. Pre-miR-34a: U2932 EBNA2 cl-1 (**) p = 0.0020 and cl-2 (**) p = 0.0027, BL41K3 (****) p < 0.0001. MiR-34a promoter: U2932 EBNA2 cl-1 (***) p = 0.0002 and cl-2 (**) p = 0.0011, BL41K3 (****) p < 0.0001