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. 2019 Jan 9;9:7. doi: 10.1038/s41598-018-37029-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Miniaturized microPADs as platforms for biochemical assays. (A) Calibration curve as generated from a colorimetric paper-based glucose assay. The data was fit with a linear trendline. Data points represent the mean of eight replicates and error bars represent one standard deviation of the mean. (B) Calibration curve for a colorimetric horseradish peroxidase (HRP) assay. The data was fit with a linear trendline. Data points represent a single replicate. (C) Photograph of the test zones after performing the HRP assays. Higher HRP concentrations produced higher color intensities in the test zones.