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. 2019 Jan 9;10:99. doi: 10.1038/s41467-018-07926-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — 3D printed high-magnification adapter reveals microvascular hemodynamics. a The microscope is shown with the 3D printed high-magnification adaptor alongside a U.S. quarter coin for scale. b Image of fluorescent dye enhanced cortical vasculature ROI. Insets are magnified fields of view from this ROI. c High-magnification mosaic stitched together from three different fields of view. Images in b, c are normalized to 1% of their intensity ranges. d Video frames from a fluorescent-dye enhanced vessel plexus from the mouse ear in which the RBCs have been highlighted in red. One can clearly visualize the motion of individual RBCs over time in each vessel segment, from which one can conduct particle velocimetry and compute the direction and magnitude of RBC flux as shown in e. Cortical and ear microvascular data presented are from two different animals, respectively. Scale bar indicates 50 μm, except in b: where it is 500 μm. Arrows indicate vessel sprouting during D6-D11. Also see Supplementary Movie 7