Figure 2.

Myeloid-Specific Knockdown of Eomes Disrupts the Expression of OC-Specific TFs

(A) Knockdown of EOMES specifically in MPs (Eomes^ΔMP/ΔMP) was confirmed by (1) DNA genotyping PCR (with historical genomic tail-tip extracted DNA as reference) and (2) western blot analysis (with quantification, n = 2) following 3 days treatment with tamoxifen using myeloid-lineage-specific Csf1rTAMCre in combination with Eomes^fl/fl mice when compared with Eomes^fl/fl, Eomes^fl/+, or Eomes^+/+ controls.

(B) Evaluation of key pro-osteoclastogenic TFs by quantitative real-time RT-PCR (n = 2–6). For all figures and subfigures, data are represented as mean ± SD for all bar graphs and all images are representative. *p < 0.05, **p < 0.01. KO, knockout; WT, wild-type.

See also Figure S2.