Figure 4.

Myeloid-Specific Eomes Deletion Decreased OC Differentiation and Function In Vivo and In Vitro

(A) Femoral micro-computed tomographic (μCT) cross-sectional images in the craniocaudal view and the distal femoral metaphysis in the proximodistal view from 8-day-old Eomes^ΔMP/ΔMP mice and Eomes^fl/fl controls. Scale bars, 1 mm.

(B) μCT quantification of bone morphometric variables (n = 3). BMD, bone mineral density; BV/TV, bone volume/total volume; Ct.Th, cortical thickness; Tb.V/AV, trabecular volume/all bone volume; Tb.N, trabecular number; Tb.Sp, trabecular spacing; Tb.Pf, trabecular pattern factor; Tb.Th, trabecular thickness.

(C) Tartrate-resistant acid phosphatase (TRAP) staining (in violet) with hematoxylin counterstain to identify OCs in the distal femoral metaphysis of mice in (A). Scale bars, 50 μm.

(D) Histomorphometric quantification of Oc.S/BS and Oc.N/BS from (C) (n = 3). Oc.N/BS, OC number per bone surface; Oc.S/BS, OC surface per bone surface.

(E and F) (E) Representative images of in vitro TRAP-positive multinucleated OC formation from Csf1rTAMCre;Eomes^fl/fl and Eomes^fl/fl mice and quantification per high-powered field (HPF) (n = 2). (F) RT-PCR analysis of genes regulating OC differentiation and function in Eomes^fl/fl (WT) MPs and OCs and Eomes^ΔMP/ΔMP (Eomes KO) OCs after differentiation (n = 2–6). *p < 0.05, **p < 0.01. KO, knockout; WT, wild-type.